destination plasmid Search Results


destination vectors  (Addgene inc)
96
Addgene inc destination vectors
Destination Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/destination+plasmid/Destination+Vector+(Plasmid+%23125754)/pmc11790949-211-0-10
Average 96 stars, based on 1 article reviews
destination vectors - by Bioz Stars, 2026-09
96/100 stars
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omega destination cmv eluc vector  (Addgene inc)
91
Addgene inc omega destination cmv eluc vector
(A) DNA parts and destination vectors (Table 1) are ordered from the nonprofit plasmid repository Addgene as bacterial stabs, streaked on bacterial plates, grown up in culture, and prepared according to Basic Protocol 1. (B) DNA parts include pathway response elements consisting of a transcription blocker coupled to transcriptional response elements that can bind transcription factors specific for cellular pathways (e.g., NF-κβ, TGF-β, MAPK/JNK, c-Myc, or p53), five orthogonal luciferases (RedF, FLuc, NLuc, Renilla, and GrRenilla), and the transcriptional terminator/polyadenylation signal from the bovine growth hormone gene (bGHpA). Note that improved TGF-β and c-Myc pathway luciferase reporters were generated that have seven canocical transcription factor binding motifs, instead of four and five as proiviously described (Sarrion-Perdigones et al., 2019). Destination vectors include five positional blue/white destination vectors for position A, B, C, D, and E, located in the final destination vector that provides a blue/white colorimetric screening marker (lacZ), and one pink/white final destination vector that provides a pink/white colorimetric screening marker (tinsel purple). (C) In the first step, novel pathway response elements are built, as described in Basic Protocol 2 or Alternate Protocol 1. (D) In the second step, novel custom luciferase reporter vectors are built in an alpha assembly, using the five positional blue/white destination vectors, pathway response element, luciferase, and transcriptional terminator, following Basic Protocol 3. (E) In the final step, a new multiplex hextuple luciferase reporter vector, consisting of four previously described pathway reporters (coupled to the luciferases RedF, FLuc, NLuc, and GrRenilla, respectively), one novel custom luciferase reporter (coupled to the luciferase Renilla in this case) and one control reporter (coupled to the luciferase <t>ELuc),</t> is generated as described in Basic Protocol 4. The same synthetic assembly pipeline can be tailored to incorporate any five previously described pathway reporters, any five novel custom luciferase reporters, or any combinations thereof, illustrating the versatility of the pipeline. (F-G) Comparison between the assembly cloning pipeline previously published stitching together 5xMyc:Renilla, 2xp53:Nluc, 4xTGFβ:FLuc, 6xMAPK:GrRenilla, <t>CMV:ELuc,</t> and 5xNFκβ:RedF luciferase reporters over five consecutive cloning reactions (Sarrion-Perdigones et al., 2019) (F), and the one presented here, stitching together 5xNFκβ:RedF, 7xTGFβ:FLuc, 3xDBE:Renilla, 2xp53:Nluc, 6xMAPK:GrRenilla, and CMV:ELuc luciferase reporters using a single cloning reaction (G), illustrating a substantial decrease in time and money investment.
Omega Destination Cmv Eluc Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/destination+plasmid/Omega+Destination-CMV%3A%3AELuc%3AbGH+(Plasmid+%23124528)/pmc07386564-917-20-23
Average 91 stars, based on 1 article reviews
omega destination cmv eluc vector - by Bioz Stars, 2026-09
91/100 stars
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ppb phosphoglycerate kinase pgk destination vector  (Addgene inc)
93
Addgene inc ppb phosphoglycerate kinase pgk destination vector
(A) DNA parts and destination vectors (Table 1) are ordered from the nonprofit plasmid repository Addgene as bacterial stabs, streaked on bacterial plates, grown up in culture, and prepared according to Basic Protocol 1. (B) DNA parts include pathway response elements consisting of a transcription blocker coupled to transcriptional response elements that can bind transcription factors specific for cellular pathways (e.g., NF-κβ, TGF-β, MAPK/JNK, c-Myc, or p53), five orthogonal luciferases (RedF, FLuc, NLuc, Renilla, and GrRenilla), and the transcriptional terminator/polyadenylation signal from the bovine growth hormone gene (bGHpA). Note that improved TGF-β and c-Myc pathway luciferase reporters were generated that have seven canocical transcription factor binding motifs, instead of four and five as proiviously described (Sarrion-Perdigones et al., 2019). Destination vectors include five positional blue/white destination vectors for position A, B, C, D, and E, located in the final destination vector that provides a blue/white colorimetric screening marker (lacZ), and one pink/white final destination vector that provides a pink/white colorimetric screening marker (tinsel purple). (C) In the first step, novel pathway response elements are built, as described in Basic Protocol 2 or Alternate Protocol 1. (D) In the second step, novel custom luciferase reporter vectors are built in an alpha assembly, using the five positional blue/white destination vectors, pathway response element, luciferase, and transcriptional terminator, following Basic Protocol 3. (E) In the final step, a new multiplex hextuple luciferase reporter vector, consisting of four previously described pathway reporters (coupled to the luciferases RedF, FLuc, NLuc, and GrRenilla, respectively), one novel custom luciferase reporter (coupled to the luciferase Renilla in this case) and one control reporter (coupled to the luciferase <t>ELuc),</t> is generated as described in Basic Protocol 4. The same synthetic assembly pipeline can be tailored to incorporate any five previously described pathway reporters, any five novel custom luciferase reporters, or any combinations thereof, illustrating the versatility of the pipeline. (F-G) Comparison between the assembly cloning pipeline previously published stitching together 5xMyc:Renilla, 2xp53:Nluc, 4xTGFβ:FLuc, 6xMAPK:GrRenilla, <t>CMV:ELuc,</t> and 5xNFκβ:RedF luciferase reporters over five consecutive cloning reactions (Sarrion-Perdigones et al., 2019) (F), and the one presented here, stitching together 5xNFκβ:RedF, 7xTGFβ:FLuc, 3xDBE:Renilla, 2xp53:Nluc, 6xMAPK:GrRenilla, and CMV:ELuc luciferase reporters using a single cloning reaction (G), illustrating a substantial decrease in time and money investment.
Ppb Phosphoglycerate Kinase Pgk Destination Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/destination+plasmid/pPB-PGK-destination+(Plasmid+%2360436)/pmc06929393-41-23-29
Average 93 stars, based on 1 article reviews
ppb phosphoglycerate kinase pgk destination vector - by Bioz Stars, 2026-09
93/100 stars
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tol2 integrable construct  (Addgene inc)
93
Addgene inc tol2 integrable construct
(A) DNA parts and destination vectors (Table 1) are ordered from the nonprofit plasmid repository Addgene as bacterial stabs, streaked on bacterial plates, grown up in culture, and prepared according to Basic Protocol 1. (B) DNA parts include pathway response elements consisting of a transcription blocker coupled to transcriptional response elements that can bind transcription factors specific for cellular pathways (e.g., NF-κβ, TGF-β, MAPK/JNK, c-Myc, or p53), five orthogonal luciferases (RedF, FLuc, NLuc, Renilla, and GrRenilla), and the transcriptional terminator/polyadenylation signal from the bovine growth hormone gene (bGHpA). Note that improved TGF-β and c-Myc pathway luciferase reporters were generated that have seven canocical transcription factor binding motifs, instead of four and five as proiviously described (Sarrion-Perdigones et al., 2019). Destination vectors include five positional blue/white destination vectors for position A, B, C, D, and E, located in the final destination vector that provides a blue/white colorimetric screening marker (lacZ), and one pink/white final destination vector that provides a pink/white colorimetric screening marker (tinsel purple). (C) In the first step, novel pathway response elements are built, as described in Basic Protocol 2 or Alternate Protocol 1. (D) In the second step, novel custom luciferase reporter vectors are built in an alpha assembly, using the five positional blue/white destination vectors, pathway response element, luciferase, and transcriptional terminator, following Basic Protocol 3. (E) In the final step, a new multiplex hextuple luciferase reporter vector, consisting of four previously described pathway reporters (coupled to the luciferases RedF, FLuc, NLuc, and GrRenilla, respectively), one novel custom luciferase reporter (coupled to the luciferase Renilla in this case) and one control reporter (coupled to the luciferase <t>ELuc),</t> is generated as described in Basic Protocol 4. The same synthetic assembly pipeline can be tailored to incorporate any five previously described pathway reporters, any five novel custom luciferase reporters, or any combinations thereof, illustrating the versatility of the pipeline. (F-G) Comparison between the assembly cloning pipeline previously published stitching together 5xMyc:Renilla, 2xp53:Nluc, 4xTGFβ:FLuc, 6xMAPK:GrRenilla, <t>CMV:ELuc,</t> and 5xNFκβ:RedF luciferase reporters over five consecutive cloning reactions (Sarrion-Perdigones et al., 2019) (F), and the one presented here, stitching together 5xNFκβ:RedF, 7xTGFβ:FLuc, 3xDBE:Renilla, 2xp53:Nluc, 6xMAPK:GrRenilla, and CMV:ELuc luciferase reporters using a single cloning reaction (G), illustrating a substantial decrease in time and money investment.
Tol2 Integrable Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/destination+plasmid/Tol2+destination+vector+(Plasmid+%23101241)/pm36739363-158-2-19
Average 93 stars, based on 1 article reviews
tol2 integrable construct - by Bioz Stars, 2026-09
93/100 stars
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pcdna3 1 destination mcs bira r118g ha  (Addgene inc)
93
Addgene inc pcdna3 1 destination mcs bira r118g ha
(A) DNA parts and destination vectors (Table 1) are ordered from the nonprofit plasmid repository Addgene as bacterial stabs, streaked on bacterial plates, grown up in culture, and prepared according to Basic Protocol 1. (B) DNA parts include pathway response elements consisting of a transcription blocker coupled to transcriptional response elements that can bind transcription factors specific for cellular pathways (e.g., NF-κβ, TGF-β, MAPK/JNK, c-Myc, or p53), five orthogonal luciferases (RedF, FLuc, NLuc, Renilla, and GrRenilla), and the transcriptional terminator/polyadenylation signal from the bovine growth hormone gene (bGHpA). Note that improved TGF-β and c-Myc pathway luciferase reporters were generated that have seven canocical transcription factor binding motifs, instead of four and five as proiviously described (Sarrion-Perdigones et al., 2019). Destination vectors include five positional blue/white destination vectors for position A, B, C, D, and E, located in the final destination vector that provides a blue/white colorimetric screening marker (lacZ), and one pink/white final destination vector that provides a pink/white colorimetric screening marker (tinsel purple). (C) In the first step, novel pathway response elements are built, as described in Basic Protocol 2 or Alternate Protocol 1. (D) In the second step, novel custom luciferase reporter vectors are built in an alpha assembly, using the five positional blue/white destination vectors, pathway response element, luciferase, and transcriptional terminator, following Basic Protocol 3. (E) In the final step, a new multiplex hextuple luciferase reporter vector, consisting of four previously described pathway reporters (coupled to the luciferases RedF, FLuc, NLuc, and GrRenilla, respectively), one novel custom luciferase reporter (coupled to the luciferase Renilla in this case) and one control reporter (coupled to the luciferase <t>ELuc),</t> is generated as described in Basic Protocol 4. The same synthetic assembly pipeline can be tailored to incorporate any five previously described pathway reporters, any five novel custom luciferase reporters, or any combinations thereof, illustrating the versatility of the pipeline. (F-G) Comparison between the assembly cloning pipeline previously published stitching together 5xMyc:Renilla, 2xp53:Nluc, 4xTGFβ:FLuc, 6xMAPK:GrRenilla, <t>CMV:ELuc,</t> and 5xNFκβ:RedF luciferase reporters over five consecutive cloning reactions (Sarrion-Perdigones et al., 2019) (F), and the one presented here, stitching together 5xNFκβ:RedF, 7xTGFβ:FLuc, 3xDBE:Renilla, 2xp53:Nluc, 6xMAPK:GrRenilla, and CMV:ELuc luciferase reporters using a single cloning reaction (G), illustrating a substantial decrease in time and money investment.
Pcdna3 1 Destination Mcs Bira R118g Ha, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/destination+plasmid/BirA(R118G)-HA+destination+vector+(Plasmid+%2353581)/pmc12824170-1-0-4
Average 93 stars, based on 1 article reviews
pcdna3 1 destination mcs bira r118g ha - by Bioz Stars, 2026-09
93/100 stars
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pcag t7 heterodimeric talen destination vectors  (Addgene inc)
90
Addgene inc pcag t7 heterodimeric talen destination vectors
<t>TALEN-based</t> generation of C57BL/6J- Prnp ZH3/ZH3 mice. (A) TALEN-binding sites within Prnp exon E3 and start codon (yellow) of the protein coding sequence. Colors indicate the code for repeat-variable diresidues. The Prnp TALEN pair incorporates second-generation <t>heterodimeric</t> FokI cleavage domains ( FokI ELD and FokI KKR ). (B) Sanger sequencing reads of a Prnp WT and a Prnp ZH3 allele from the founder F 0 mouse. A deletion of 8 bp in the Prnp ZH3 allele (highlighted by a red box on the WT sequence) introduces a T/D residue change, followed by a premature STOP codon (*) after residue 14 within the sequence encoding the PrP C signal peptide. The deletion also eliminates the Tsp45I recognition sequence (blue letters on WT sequence). As a result of sequence characteristics in this region, an alternative 8-bp deletion (ACTATGTG), shifted by 4 bp in respect to the previous deletion, is also compatible with the generation of the Prnp ZH3 allele. (C) Representative image of routinely used RFLP analysis discriminating Prnp WT/WT (digested amplicons), Prnp WT/ZH3 (digested and undigested amplicons), and Prnp ZH3/ZH3 mice (only undigested amplicon). Primers location, restriction site, and expected sizes for digestion products are indicated on top of the gel image. (D) Allelic discrimination genotyping using a FAM-labeled WT-specific probe and a Yakima Yellow–labeled ZH3-specific probe. NTC, no-template control. ΔRn, difference in normalized reporter fluorescence after and before amplification. Apart from NTC, each triangle denotes one mouse ( n = 4 mice/genotype). The mean (triangle) and SD (blue error bars) for four technical replicates of each mouse/NTC sample are shown. (E) Immunoblot analysis of PrP C expression in different CNS regions (Cx, cortex; Sc, spinal cord; Cb, cerebellum) of Prnp WT/WT (WT) and Prnp ZH3/ZH3 (ZH3) mice was performed using POM1 (against helices α1 and α3 of the PrP C globular domain). The blot was also decorated with anti-actin antibody as control. (F) Brain PrP C levels as determined by sandwich POM1-POM2 ELISA. Prnp Edbg/Edbg (Edbg) served as negative controls. Each circle denotes a mouse ( n = 3 mice/genotype). Horizontal bar indicates mean. WT→KO, consecutive log 2 dilutions of Prnp WT/WT into Prnp Edbg/Edbg homogenate, indicating that the threshold of detectability was 1:16. (G) Immunofluorescence staining of cerebelli. MAP2 is displayed in green, PrP C , detected with POM19 (against helices β1 and α3 of globular domain) in red, and DAPI in blue. Bar, 20 µm. (D–G) Representative data from two independent experiments.
Pcag T7 Heterodimeric Talen Destination Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/destination+plasmid/pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination+(Plasmid+%2340131)/pmc04813672-122-19-28
Average 90 stars, based on 1 article reviews
pcag t7 heterodimeric talen destination vectors - by Bioz Stars, 2026-09
90/100 stars
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pet empty polycistronic destination vector 2e  (Addgene inc)
86
Addgene inc pet empty polycistronic destination vector 2e
<t>TALEN-based</t> generation of C57BL/6J- Prnp ZH3/ZH3 mice. (A) TALEN-binding sites within Prnp exon E3 and start codon (yellow) of the protein coding sequence. Colors indicate the code for repeat-variable diresidues. The Prnp TALEN pair incorporates second-generation <t>heterodimeric</t> FokI cleavage domains ( FokI ELD and FokI KKR ). (B) Sanger sequencing reads of a Prnp WT and a Prnp ZH3 allele from the founder F 0 mouse. A deletion of 8 bp in the Prnp ZH3 allele (highlighted by a red box on the WT sequence) introduces a T/D residue change, followed by a premature STOP codon (*) after residue 14 within the sequence encoding the PrP C signal peptide. The deletion also eliminates the Tsp45I recognition sequence (blue letters on WT sequence). As a result of sequence characteristics in this region, an alternative 8-bp deletion (ACTATGTG), shifted by 4 bp in respect to the previous deletion, is also compatible with the generation of the Prnp ZH3 allele. (C) Representative image of routinely used RFLP analysis discriminating Prnp WT/WT (digested amplicons), Prnp WT/ZH3 (digested and undigested amplicons), and Prnp ZH3/ZH3 mice (only undigested amplicon). Primers location, restriction site, and expected sizes for digestion products are indicated on top of the gel image. (D) Allelic discrimination genotyping using a FAM-labeled WT-specific probe and a Yakima Yellow–labeled ZH3-specific probe. NTC, no-template control. ΔRn, difference in normalized reporter fluorescence after and before amplification. Apart from NTC, each triangle denotes one mouse ( n = 4 mice/genotype). The mean (triangle) and SD (blue error bars) for four technical replicates of each mouse/NTC sample are shown. (E) Immunoblot analysis of PrP C expression in different CNS regions (Cx, cortex; Sc, spinal cord; Cb, cerebellum) of Prnp WT/WT (WT) and Prnp ZH3/ZH3 (ZH3) mice was performed using POM1 (against helices α1 and α3 of the PrP C globular domain). The blot was also decorated with anti-actin antibody as control. (F) Brain PrP C levels as determined by sandwich POM1-POM2 ELISA. Prnp Edbg/Edbg (Edbg) served as negative controls. Each circle denotes a mouse ( n = 3 mice/genotype). Horizontal bar indicates mean. WT→KO, consecutive log 2 dilutions of Prnp WT/WT into Prnp Edbg/Edbg homogenate, indicating that the threshold of detectability was 1:16. (G) Immunofluorescence staining of cerebelli. MAP2 is displayed in green, PrP C , detected with POM19 (against helices β1 and α3 of globular domain) in red, and DAPI in blue. Bar, 20 µm. (D–G) Representative data from two independent experiments.
Pet Empty Polycistronic Destination Vector 2e, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/destination+plasmid/pET+empty+polycistronic+destination+vector+(2E)+(Plasmid+%2329775)/pmc05562863-266-53-59
Average 86 stars, based on 1 article reviews
pet empty polycistronic destination vector 2e - by Bioz Stars, 2026-09
86/100 stars
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destination vector pcag t7 talen  (Addgene inc)
92
Addgene inc destination vector pcag t7 talen
<t>TALEN-based</t> generation of C57BL/6J- Prnp ZH3/ZH3 mice. (A) TALEN-binding sites within Prnp exon E3 and start codon (yellow) of the protein coding sequence. Colors indicate the code for repeat-variable diresidues. The Prnp TALEN pair incorporates second-generation <t>heterodimeric</t> FokI cleavage domains ( FokI ELD and FokI KKR ). (B) Sanger sequencing reads of a Prnp WT and a Prnp ZH3 allele from the founder F 0 mouse. A deletion of 8 bp in the Prnp ZH3 allele (highlighted by a red box on the WT sequence) introduces a T/D residue change, followed by a premature STOP codon (*) after residue 14 within the sequence encoding the PrP C signal peptide. The deletion also eliminates the Tsp45I recognition sequence (blue letters on WT sequence). As a result of sequence characteristics in this region, an alternative 8-bp deletion (ACTATGTG), shifted by 4 bp in respect to the previous deletion, is also compatible with the generation of the Prnp ZH3 allele. (C) Representative image of routinely used RFLP analysis discriminating Prnp WT/WT (digested amplicons), Prnp WT/ZH3 (digested and undigested amplicons), and Prnp ZH3/ZH3 mice (only undigested amplicon). Primers location, restriction site, and expected sizes for digestion products are indicated on top of the gel image. (D) Allelic discrimination genotyping using a FAM-labeled WT-specific probe and a Yakima Yellow–labeled ZH3-specific probe. NTC, no-template control. ΔRn, difference in normalized reporter fluorescence after and before amplification. Apart from NTC, each triangle denotes one mouse ( n = 4 mice/genotype). The mean (triangle) and SD (blue error bars) for four technical replicates of each mouse/NTC sample are shown. (E) Immunoblot analysis of PrP C expression in different CNS regions (Cx, cortex; Sc, spinal cord; Cb, cerebellum) of Prnp WT/WT (WT) and Prnp ZH3/ZH3 (ZH3) mice was performed using POM1 (against helices α1 and α3 of the PrP C globular domain). The blot was also decorated with anti-actin antibody as control. (F) Brain PrP C levels as determined by sandwich POM1-POM2 ELISA. Prnp Edbg/Edbg (Edbg) served as negative controls. Each circle denotes a mouse ( n = 3 mice/genotype). Horizontal bar indicates mean. WT→KO, consecutive log 2 dilutions of Prnp WT/WT into Prnp Edbg/Edbg homogenate, indicating that the threshold of detectability was 1:16. (G) Immunofluorescence staining of cerebelli. MAP2 is displayed in green, PrP C , detected with POM19 (against helices β1 and α3 of globular domain) in red, and DAPI in blue. Bar, 20 µm. (D–G) Representative data from two independent experiments.
Destination Vector Pcag T7 Talen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/destination+plasmid/pCAG-T7-TALEN(Sangamo)-Destination+(Plasmid+%2337184)/pmc05014520-174-20-23
Average 92 stars, based on 1 article reviews
destination vector pcag t7 talen - by Bioz Stars, 2026-09
92/100 stars
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destination  (Addgene inc)
92
Addgene inc destination
iCre/Lox toolkit components available at Addgene.
Destination, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/destination+plasmid/pPB-CAG-destination+(Plasmid+%2360437)/pmc10433388-79-24-27
Average 92 stars, based on 1 article reviews
destination - by Bioz Stars, 2026-09
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destination plasmid  (Promega)
90
Promega destination plasmid
iCre/Lox toolkit components available at Addgene.
Destination Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/destination+plasmid/destination+plasmid/us08691505-157-64-20
Average 90 stars, based on 1 article reviews
destination plasmid - by Bioz Stars, 2026-09
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destination plasmid  (Addgene inc)
93
Addgene inc destination plasmid
iCre/Lox toolkit components available at Addgene.
Destination Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/destination+plasmid/LII+destination+(Plasmid+%23104529)/pm24704356-51-35-61
Average 93 stars, based on 1 article reviews
destination plasmid - by Bioz Stars, 2026-09
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destination vector pet 2z  (Addgene inc)
90
Addgene inc destination vector pet 2z
iCre/Lox toolkit components available at Addgene.
Destination Vector Pet 2z, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/destination+plasmid/pET+empty+polycistronic+destination+vector+(2Z)+(Plasmid+%2329776)/pm24932924-108-1-6
Average 90 stars, based on 1 article reviews
destination vector pet 2z - by Bioz Stars, 2026-09
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Image Search Results


(A) DNA parts and destination vectors (Table 1) are ordered from the nonprofit plasmid repository Addgene as bacterial stabs, streaked on bacterial plates, grown up in culture, and prepared according to Basic Protocol 1. (B) DNA parts include pathway response elements consisting of a transcription blocker coupled to transcriptional response elements that can bind transcription factors specific for cellular pathways (e.g., NF-κβ, TGF-β, MAPK/JNK, c-Myc, or p53), five orthogonal luciferases (RedF, FLuc, NLuc, Renilla, and GrRenilla), and the transcriptional terminator/polyadenylation signal from the bovine growth hormone gene (bGHpA). Note that improved TGF-β and c-Myc pathway luciferase reporters were generated that have seven canocical transcription factor binding motifs, instead of four and five as proiviously described (Sarrion-Perdigones et al., 2019). Destination vectors include five positional blue/white destination vectors for position A, B, C, D, and E, located in the final destination vector that provides a blue/white colorimetric screening marker (lacZ), and one pink/white final destination vector that provides a pink/white colorimetric screening marker (tinsel purple). (C) In the first step, novel pathway response elements are built, as described in Basic Protocol 2 or Alternate Protocol 1. (D) In the second step, novel custom luciferase reporter vectors are built in an alpha assembly, using the five positional blue/white destination vectors, pathway response element, luciferase, and transcriptional terminator, following Basic Protocol 3. (E) In the final step, a new multiplex hextuple luciferase reporter vector, consisting of four previously described pathway reporters (coupled to the luciferases RedF, FLuc, NLuc, and GrRenilla, respectively), one novel custom luciferase reporter (coupled to the luciferase Renilla in this case) and one control reporter (coupled to the luciferase ELuc), is generated as described in Basic Protocol 4. The same synthetic assembly pipeline can be tailored to incorporate any five previously described pathway reporters, any five novel custom luciferase reporters, or any combinations thereof, illustrating the versatility of the pipeline. (F-G) Comparison between the assembly cloning pipeline previously published stitching together 5xMyc:Renilla, 2xp53:Nluc, 4xTGFβ:FLuc, 6xMAPK:GrRenilla, CMV:ELuc, and 5xNFκβ:RedF luciferase reporters over five consecutive cloning reactions (Sarrion-Perdigones et al., 2019) (F), and the one presented here, stitching together 5xNFκβ:RedF, 7xTGFβ:FLuc, 3xDBE:Renilla, 2xp53:Nluc, 6xMAPK:GrRenilla, and CMV:ELuc luciferase reporters using a single cloning reaction (G), illustrating a substantial decrease in time and money investment.

Journal: Current protocols in molecular biology

Article Title: Rapid and efficient synthetic assembly of multiplex luciferase reporter plasmids for the simultaneous monitoring of up to six cellular signaling pathways

doi: 10.1002/cpmb.121

Figure Lengend Snippet: (A) DNA parts and destination vectors (Table 1) are ordered from the nonprofit plasmid repository Addgene as bacterial stabs, streaked on bacterial plates, grown up in culture, and prepared according to Basic Protocol 1. (B) DNA parts include pathway response elements consisting of a transcription blocker coupled to transcriptional response elements that can bind transcription factors specific for cellular pathways (e.g., NF-κβ, TGF-β, MAPK/JNK, c-Myc, or p53), five orthogonal luciferases (RedF, FLuc, NLuc, Renilla, and GrRenilla), and the transcriptional terminator/polyadenylation signal from the bovine growth hormone gene (bGHpA). Note that improved TGF-β and c-Myc pathway luciferase reporters were generated that have seven canocical transcription factor binding motifs, instead of four and five as proiviously described (Sarrion-Perdigones et al., 2019). Destination vectors include five positional blue/white destination vectors for position A, B, C, D, and E, located in the final destination vector that provides a blue/white colorimetric screening marker (lacZ), and one pink/white final destination vector that provides a pink/white colorimetric screening marker (tinsel purple). (C) In the first step, novel pathway response elements are built, as described in Basic Protocol 2 or Alternate Protocol 1. (D) In the second step, novel custom luciferase reporter vectors are built in an alpha assembly, using the five positional blue/white destination vectors, pathway response element, luciferase, and transcriptional terminator, following Basic Protocol 3. (E) In the final step, a new multiplex hextuple luciferase reporter vector, consisting of four previously described pathway reporters (coupled to the luciferases RedF, FLuc, NLuc, and GrRenilla, respectively), one novel custom luciferase reporter (coupled to the luciferase Renilla in this case) and one control reporter (coupled to the luciferase ELuc), is generated as described in Basic Protocol 4. The same synthetic assembly pipeline can be tailored to incorporate any five previously described pathway reporters, any five novel custom luciferase reporters, or any combinations thereof, illustrating the versatility of the pipeline. (F-G) Comparison between the assembly cloning pipeline previously published stitching together 5xMyc:Renilla, 2xp53:Nluc, 4xTGFβ:FLuc, 6xMAPK:GrRenilla, CMV:ELuc, and 5xNFκβ:RedF luciferase reporters over five consecutive cloning reactions (Sarrion-Perdigones et al., 2019) (F), and the one presented here, stitching together 5xNFκβ:RedF, 7xTGFβ:FLuc, 3xDBE:Renilla, 2xp53:Nluc, 6xMAPK:GrRenilla, and CMV:ELuc luciferase reporters using a single cloning reaction (G), illustrating a substantial decrease in time and money investment.

Article Snippet: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Prepare the ligation mix in a 0.2 mL PCR tube by pipetting: 75 ng of the Omega destination-CMV:ELuc vector (Addgene #124528).

Techniques: Plasmid Preparation, Luciferase, Generated, Binding Assay, Marker, Multiplex Assay, Clone Assay

Summary of vectors described in this work.

Journal: Current protocols in molecular biology

Article Title: Rapid and efficient synthetic assembly of multiplex luciferase reporter plasmids for the simultaneous monitoring of up to six cellular signaling pathways

doi: 10.1002/cpmb.121

Figure Lengend Snippet: Summary of vectors described in this work.

Article Snippet: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Prepare the ligation mix in a 0.2 mL PCR tube by pipetting: 75 ng of the Omega destination-CMV:ELuc vector (Addgene #124528).

Techniques: Plasmid Preparation, Luciferase, Binding Assay, Multiplex Assay

Restriction Enzyme mixes and expected fragment sizes

Journal: Current protocols in molecular biology

Article Title: Rapid and efficient synthetic assembly of multiplex luciferase reporter plasmids for the simultaneous monitoring of up to six cellular signaling pathways

doi: 10.1002/cpmb.121

Figure Lengend Snippet: Restriction Enzyme mixes and expected fragment sizes

Article Snippet: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Prepare the ligation mix in a 0.2 mL PCR tube by pipetting: 75 ng of the Omega destination-CMV:ELuc vector (Addgene #124528).

Techniques: Plasmid Preparation

Purified plasmid DNA is analyzed by agarose gel electrophoresis two-ways: plasmid fingerprinting after restriction enzyme cutting (to confirm appropriate DNA banding patterns) and uncut (to confirm the absence of unwanted multimerizations). (A) Quality control of domesticator plasmid pUPD3 (#1), destination plasmids AlphaA (#2), AlphaC (#3), AlphaB (#4), AlphaD (#5), and AlphaE (#6), and final destination plasmid Omega-CMV:Eluc (#7). (B) Quality control of luciferase plasmids pFLuc (#8), pRedF (#9), pNLuc (#10) pRenilla (#11), and pGrRenilla (#12), and the transcriptional terminator plasmid pbGH (#13). (C) Quality control of pathway response element plasmids TB:5xNF-κβ_RE:MiniP (#14), TB:7xSMAD_RE:MiniP (#15), TB:7xE-Box:MiniP (#16), TB:2xP53_RE:MiniP (#17), and TB:6xAP-1_RE:MiniP (#18). (D) Quality control of transcriptional response luciferase reporter plasmids 5xNF-κβ_RE::RedF (#19), 7xSMAD_RE::FLuc (#20), 7xE-Box::Renilla (#21), 2xP53_RE::NLuc (#22), and 6xAP-1_RE::GrRenilla (#23).

Journal: Current protocols in molecular biology

Article Title: Rapid and efficient synthetic assembly of multiplex luciferase reporter plasmids for the simultaneous monitoring of up to six cellular signaling pathways

doi: 10.1002/cpmb.121

Figure Lengend Snippet: Purified plasmid DNA is analyzed by agarose gel electrophoresis two-ways: plasmid fingerprinting after restriction enzyme cutting (to confirm appropriate DNA banding patterns) and uncut (to confirm the absence of unwanted multimerizations). (A) Quality control of domesticator plasmid pUPD3 (#1), destination plasmids AlphaA (#2), AlphaC (#3), AlphaB (#4), AlphaD (#5), and AlphaE (#6), and final destination plasmid Omega-CMV:Eluc (#7). (B) Quality control of luciferase plasmids pFLuc (#8), pRedF (#9), pNLuc (#10) pRenilla (#11), and pGrRenilla (#12), and the transcriptional terminator plasmid pbGH (#13). (C) Quality control of pathway response element plasmids TB:5xNF-κβ_RE:MiniP (#14), TB:7xSMAD_RE:MiniP (#15), TB:7xE-Box:MiniP (#16), TB:2xP53_RE:MiniP (#17), and TB:6xAP-1_RE:MiniP (#18). (D) Quality control of transcriptional response luciferase reporter plasmids 5xNF-κβ_RE::RedF (#19), 7xSMAD_RE::FLuc (#20), 7xE-Box::Renilla (#21), 2xP53_RE::NLuc (#22), and 6xAP-1_RE::GrRenilla (#23).

Article Snippet: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Prepare the ligation mix in a 0.2 mL PCR tube by pipetting: 75 ng of the Omega destination-CMV:ELuc vector (Addgene #124528).

Techniques: Purification, Plasmid Preparation, Agarose Gel Electrophoresis, Luciferase

(A) Overview of the in silico scarless assembly of a multiplex luciferase reporter in the destination vector Omega Destination-CMV:ELuc:bGH. Five “transcriptional reporter” plasmids, AlphaA 5xNF-KB:RedF:bGHpA (Addgene #124530) reporting on NF-κβ pathway signaling using red firefly luciferase (RF), AlphaB 7xSMAD:FLuc:bGHpA (Addgene #124531) reporting on TGF-β signaling using firefly luciferase (FL), AlphaC 3xDBE:Renilla:bGHpA (Addgene #124535) reporting on FoxO pathway signaling using renilla luciferase (Re), AlphaD 2xp53:NLuc:bGHpA (Addgene #124533) reporting on p53 pathway signaling using nano luciferase (NL), and AlphaE 6xAP1_RE:GrRenilla:bGHpA (Addgene #124534) reporting on MAPK/JNK pathway signaling using green renilla luciferase (GR), as well as the destination vector Omega Destination-CMV:ELuc:bGH containing the control enhanced beetle luciferase (EL) for normalization purposes are incubated together with BsmBI and T4 DNA ligase. Correct multipartitie stitching of all five BsmBI-released transcriptional luciferase reporter units into BsmBI-opended Omega Destination-CMV:ELuc:bGH, results in the final multiplex luciferase vector MLRV2:NF-kb-SMAD-DBE-P53-AP1 (Addgene #124536), consisting of five transcriptional luciferase reporter units stitched together in a specified order, and one control luciferase reporter unit (constitutively expressed ELuc luciferase). Assembled plasmids are identified as white colored colonies that are characterized further (see C), while religated Omega Destination-CMV:ELuc:bGH plasmids are pink to purple colored due to the presence of the colorimetric marker, tinsel purple. (B) Overview of the cloning reaction. Prepare a reaction mix containing 75 ng of the final pink/white destination vector (Omega Destination-CMV:ELuc:bGH), 75 ng of each of the luciferase entry vectors, the type IIs restriction enzyme BsmBI, T4 DNA ligase and 10x T4 DNA ligase buffer. Start the assembly protocol that cycles 25 times between 37°C and 16°C. (C) Extended assembly cycling reaction conditions, with 50 cycles of 37°C and 16°C, followed by 1h at 37°C to favor digestion of uncut plasmids, 20 minutes at 85°C to denature the enzymes and a prolonged incubation at 16°C. (D) Restriction enzyme digestion of 3 white colored colonies using ScaI (6510, 2219, 1790, 1543, 1088 and 486 bps) and XhoI (6500, 2263, 1508, 1098, 979, 794 and 494 bps), and uncut DNA. All colonies show the correct digestion pattern.

Journal: Current protocols in molecular biology

Article Title: Rapid and efficient synthetic assembly of multiplex luciferase reporter plasmids for the simultaneous monitoring of up to six cellular signaling pathways

doi: 10.1002/cpmb.121

Figure Lengend Snippet: (A) Overview of the in silico scarless assembly of a multiplex luciferase reporter in the destination vector Omega Destination-CMV:ELuc:bGH. Five “transcriptional reporter” plasmids, AlphaA 5xNF-KB:RedF:bGHpA (Addgene #124530) reporting on NF-κβ pathway signaling using red firefly luciferase (RF), AlphaB 7xSMAD:FLuc:bGHpA (Addgene #124531) reporting on TGF-β signaling using firefly luciferase (FL), AlphaC 3xDBE:Renilla:bGHpA (Addgene #124535) reporting on FoxO pathway signaling using renilla luciferase (Re), AlphaD 2xp53:NLuc:bGHpA (Addgene #124533) reporting on p53 pathway signaling using nano luciferase (NL), and AlphaE 6xAP1_RE:GrRenilla:bGHpA (Addgene #124534) reporting on MAPK/JNK pathway signaling using green renilla luciferase (GR), as well as the destination vector Omega Destination-CMV:ELuc:bGH containing the control enhanced beetle luciferase (EL) for normalization purposes are incubated together with BsmBI and T4 DNA ligase. Correct multipartitie stitching of all five BsmBI-released transcriptional luciferase reporter units into BsmBI-opended Omega Destination-CMV:ELuc:bGH, results in the final multiplex luciferase vector MLRV2:NF-kb-SMAD-DBE-P53-AP1 (Addgene #124536), consisting of five transcriptional luciferase reporter units stitched together in a specified order, and one control luciferase reporter unit (constitutively expressed ELuc luciferase). Assembled plasmids are identified as white colored colonies that are characterized further (see C), while religated Omega Destination-CMV:ELuc:bGH plasmids are pink to purple colored due to the presence of the colorimetric marker, tinsel purple. (B) Overview of the cloning reaction. Prepare a reaction mix containing 75 ng of the final pink/white destination vector (Omega Destination-CMV:ELuc:bGH), 75 ng of each of the luciferase entry vectors, the type IIs restriction enzyme BsmBI, T4 DNA ligase and 10x T4 DNA ligase buffer. Start the assembly protocol that cycles 25 times between 37°C and 16°C. (C) Extended assembly cycling reaction conditions, with 50 cycles of 37°C and 16°C, followed by 1h at 37°C to favor digestion of uncut plasmids, 20 minutes at 85°C to denature the enzymes and a prolonged incubation at 16°C. (D) Restriction enzyme digestion of 3 white colored colonies using ScaI (6510, 2219, 1790, 1543, 1088 and 486 bps) and XhoI (6500, 2263, 1508, 1098, 979, 794 and 494 bps), and uncut DNA. All colonies show the correct digestion pattern.

Article Snippet: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Prepare the ligation mix in a 0.2 mL PCR tube by pipetting: 75 ng of the Omega destination-CMV:ELuc vector (Addgene #124528).

Techniques: In Silico, Multiplex Assay, Luciferase, Plasmid Preparation, Incubation, Marker, Clone Assay

TALEN-based generation of C57BL/6J- Prnp ZH3/ZH3 mice. (A) TALEN-binding sites within Prnp exon E3 and start codon (yellow) of the protein coding sequence. Colors indicate the code for repeat-variable diresidues. The Prnp TALEN pair incorporates second-generation heterodimeric FokI cleavage domains ( FokI ELD and FokI KKR ). (B) Sanger sequencing reads of a Prnp WT and a Prnp ZH3 allele from the founder F 0 mouse. A deletion of 8 bp in the Prnp ZH3 allele (highlighted by a red box on the WT sequence) introduces a T/D residue change, followed by a premature STOP codon (*) after residue 14 within the sequence encoding the PrP C signal peptide. The deletion also eliminates the Tsp45I recognition sequence (blue letters on WT sequence). As a result of sequence characteristics in this region, an alternative 8-bp deletion (ACTATGTG), shifted by 4 bp in respect to the previous deletion, is also compatible with the generation of the Prnp ZH3 allele. (C) Representative image of routinely used RFLP analysis discriminating Prnp WT/WT (digested amplicons), Prnp WT/ZH3 (digested and undigested amplicons), and Prnp ZH3/ZH3 mice (only undigested amplicon). Primers location, restriction site, and expected sizes for digestion products are indicated on top of the gel image. (D) Allelic discrimination genotyping using a FAM-labeled WT-specific probe and a Yakima Yellow–labeled ZH3-specific probe. NTC, no-template control. ΔRn, difference in normalized reporter fluorescence after and before amplification. Apart from NTC, each triangle denotes one mouse ( n = 4 mice/genotype). The mean (triangle) and SD (blue error bars) for four technical replicates of each mouse/NTC sample are shown. (E) Immunoblot analysis of PrP C expression in different CNS regions (Cx, cortex; Sc, spinal cord; Cb, cerebellum) of Prnp WT/WT (WT) and Prnp ZH3/ZH3 (ZH3) mice was performed using POM1 (against helices α1 and α3 of the PrP C globular domain). The blot was also decorated with anti-actin antibody as control. (F) Brain PrP C levels as determined by sandwich POM1-POM2 ELISA. Prnp Edbg/Edbg (Edbg) served as negative controls. Each circle denotes a mouse ( n = 3 mice/genotype). Horizontal bar indicates mean. WT→KO, consecutive log 2 dilutions of Prnp WT/WT into Prnp Edbg/Edbg homogenate, indicating that the threshold of detectability was 1:16. (G) Immunofluorescence staining of cerebelli. MAP2 is displayed in green, PrP C , detected with POM19 (against helices β1 and α3 of globular domain) in red, and DAPI in blue. Bar, 20 µm. (D–G) Representative data from two independent experiments.

Journal: The Journal of Experimental Medicine

Article Title: Strictly co-isogenic C57BL/6J- Prnp −/− mice: A rigorous resource for prion science

doi: 10.1084/jem.20151610

Figure Lengend Snippet: TALEN-based generation of C57BL/6J- Prnp ZH3/ZH3 mice. (A) TALEN-binding sites within Prnp exon E3 and start codon (yellow) of the protein coding sequence. Colors indicate the code for repeat-variable diresidues. The Prnp TALEN pair incorporates second-generation heterodimeric FokI cleavage domains ( FokI ELD and FokI KKR ). (B) Sanger sequencing reads of a Prnp WT and a Prnp ZH3 allele from the founder F 0 mouse. A deletion of 8 bp in the Prnp ZH3 allele (highlighted by a red box on the WT sequence) introduces a T/D residue change, followed by a premature STOP codon (*) after residue 14 within the sequence encoding the PrP C signal peptide. The deletion also eliminates the Tsp45I recognition sequence (blue letters on WT sequence). As a result of sequence characteristics in this region, an alternative 8-bp deletion (ACTATGTG), shifted by 4 bp in respect to the previous deletion, is also compatible with the generation of the Prnp ZH3 allele. (C) Representative image of routinely used RFLP analysis discriminating Prnp WT/WT (digested amplicons), Prnp WT/ZH3 (digested and undigested amplicons), and Prnp ZH3/ZH3 mice (only undigested amplicon). Primers location, restriction site, and expected sizes for digestion products are indicated on top of the gel image. (D) Allelic discrimination genotyping using a FAM-labeled WT-specific probe and a Yakima Yellow–labeled ZH3-specific probe. NTC, no-template control. ΔRn, difference in normalized reporter fluorescence after and before amplification. Apart from NTC, each triangle denotes one mouse ( n = 4 mice/genotype). The mean (triangle) and SD (blue error bars) for four technical replicates of each mouse/NTC sample are shown. (E) Immunoblot analysis of PrP C expression in different CNS regions (Cx, cortex; Sc, spinal cord; Cb, cerebellum) of Prnp WT/WT (WT) and Prnp ZH3/ZH3 (ZH3) mice was performed using POM1 (against helices α1 and α3 of the PrP C globular domain). The blot was also decorated with anti-actin antibody as control. (F) Brain PrP C levels as determined by sandwich POM1-POM2 ELISA. Prnp Edbg/Edbg (Edbg) served as negative controls. Each circle denotes a mouse ( n = 3 mice/genotype). Horizontal bar indicates mean. WT→KO, consecutive log 2 dilutions of Prnp WT/WT into Prnp Edbg/Edbg homogenate, indicating that the threshold of detectability was 1:16. (G) Immunofluorescence staining of cerebelli. MAP2 is displayed in green, PrP C , detected with POM19 (against helices β1 and α3 of globular domain) in red, and DAPI in blue. Bar, 20 µm. (D–G) Representative data from two independent experiments.

Article Snippet: TALENs were assembled using the Golden Gate TALEN and TAL Effector kit (plasmid kit 1000000024; Addgene; ) and the pCAG-T7 heterodimeric TALEN destination vectors (plasmids 40131 and 40132; Addgene; ).

Techniques: Binding Assay, Sequencing, Residue, Amplification, Labeling, Control, Fluorescence, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining

C57BL/6- Prnp ZH3/ZH3 mice do not have TALEN off-target cleavage sites. T7 endonuclease I digestion of PCR products generated from predicted OTs ( Table S1 ) and Prnp as a digestion positive control. Analyses were performed on the founder Prnp WT/ZH3 mouse and one control C57BL/6J mouse. Before enzymatic digestion, amplicons were subjected to a temperature gradient enabling the formation of heteroduplexes in the presence of heterozygous mutations in the amplified gDNA. In the presence of TALEN-induced mutations, fragments of the size indicated below the gels are expected to appear, in addition to the undigested, WT amplicon. Nonconsecutive lanes from the same gel show Prnp amplicon as a control. Only in the founder Prnp WT/ZH3 mouse T7 endonuclease I digestion of the Prnp amplicon results in the formation of the two predicted fragments (indicated by an asterisk).

Journal: The Journal of Experimental Medicine

Article Title: Strictly co-isogenic C57BL/6J- Prnp −/− mice: A rigorous resource for prion science

doi: 10.1084/jem.20151610

Figure Lengend Snippet: C57BL/6- Prnp ZH3/ZH3 mice do not have TALEN off-target cleavage sites. T7 endonuclease I digestion of PCR products generated from predicted OTs ( Table S1 ) and Prnp as a digestion positive control. Analyses were performed on the founder Prnp WT/ZH3 mouse and one control C57BL/6J mouse. Before enzymatic digestion, amplicons were subjected to a temperature gradient enabling the formation of heteroduplexes in the presence of heterozygous mutations in the amplified gDNA. In the presence of TALEN-induced mutations, fragments of the size indicated below the gels are expected to appear, in addition to the undigested, WT amplicon. Nonconsecutive lanes from the same gel show Prnp amplicon as a control. Only in the founder Prnp WT/ZH3 mouse T7 endonuclease I digestion of the Prnp amplicon results in the formation of the two predicted fragments (indicated by an asterisk).

Article Snippet: TALENs were assembled using the Golden Gate TALEN and TAL Effector kit (plasmid kit 1000000024; Addgene; ) and the pCAG-T7 heterodimeric TALEN destination vectors (plasmids 40131 and 40132; Addgene; ).

Techniques: Generated, Positive Control, Control, Amplification

iCre/Lox toolkit components available at Addgene.

Journal: Frontiers in Physiology

Article Title: Optimising the zebrafish Cre/Lox toolbox. Codon improved iCre, new gateway tools, Cre protein and guidelines

doi: 10.3389/fphys.2023.1221310

Figure Lengend Snippet: iCre/Lox toolkit components available at Addgene.

Article Snippet: To further validate our material and iCre’s efficiency in transgenic experiments, we recombined 469-pME-iCre with p5E-UBI (5′clone containing the Ubiquitin promoter) into the optimized destination clone 1456-pDEST-miniTol2_R4R2_Cryst-eGFP (Addgene # 171795), and injected this final construct 114-UBI:iCre_crystGFP (Addgene #171797) with tol2 transposase to promote genomic integration ( ; ).

Techniques: Sequencing, In Vivo, Expressing, Plasmid Preparation, Construct, Clone Assay, Cloning, Marker, Control, Ubiquitin Proteomics